sequence encoding ecfp frb  (Addgene inc)

Journal: Nature methods

Article Title: A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity.

doi: 10.1038/s41592-023-01988-8

Figure Lengend Snippet: Fig. 2 | CATCHFIRE enables the control of protein dimerization with high temporal control. a–f, HeLa cells coexpressing mCherry–FIREtag and FIREmate– eCFP–Giantin (a,b), FIREmate–eCFP–Cb5 (c,d), Lyn11–FIREmate–eCFP (e,f) were treated with 10 μM match550 and imaged by timelapse confocal microscopy. Representative confocal micrographs of cells before (0 min) and after (5 min) addition of match550 (Supplementary Videos 9–11) (a,c,e). Experiments were repeated three times with similar results. Temporal evolution of the CATCHFIRE signal (b,d,f). Data represent the mean ± s.d. of three independent experiments (n = 11 cells (b), 15 cells (d) and 15 cells (f)). g,h, HeLa cells coexpressing mCherry–FIREtag and Giantin–IRFP–FIREmate were treated with 10 μM match550 for 240 s, washed for 212 s and then treated with 10 μM match540 for 212 s and washed

Article Snippet: The plasmid pAG1171 for the expression of FIREmate–eCFP– Giantin was constructed by replacing the sequence coding for FRB– eCFP(W66A)–Giantin by FIREmate–eCFP sequence in the Addgene vector (#67903) coding for FRB–eCFP(W66A)–Giantin16.

Techniques: Control, Confocal Microscopy

Journal: Nature methods

Article Title: A fluorogenic chemically induced dimerization technology for controlling, imaging and sensing protein proximity.

doi: 10.1038/s41592-023-01988-8

Figure Lengend Snippet: Fig. 2 | CATCHFIRE enables the control of protein dimerization with high temporal control. a–f, HeLa cells coexpressing mCherry–FIREtag and FIREmate– eCFP–Giantin (a,b), FIREmate–eCFP–Cb5 (c,d), Lyn11–FIREmate–eCFP (e,f) were treated with 10 μM match550 and imaged by timelapse confocal microscopy. Representative confocal micrographs of cells before (0 min) and after (5 min) addition of match550 (Supplementary Videos 9–11) (a,c,e). Experiments were repeated three times with similar results. Temporal evolution of the CATCHFIRE signal (b,d,f). Data represent the mean ± s.d. of three independent experiments (n = 11 cells (b), 15 cells (d) and 15 cells (f)). g,h, HeLa cells coexpressing mCherry–FIREtag and Giantin–IRFP–FIREmate were treated with 10 μM match550 for 240 s, washed for 212 s and then treated with 10 μM match540 for 212 s and washed

Article Snippet: The plasmid pAG1211 for the expression of FRB–eCFP–Giantin was constructed by replacing the sequence coding for eCFP(W66A) by the eCFP sequence in the Addgene vector (#67903) coding for FRB–eCFP(W66A)–Giantin16.

Techniques: Control, Confocal Microscopy